Production of Pasteurella pestis toxin.
نویسندگان
چکیده
In the course of the development of a casein hydrolyzate mineral glucose medium for the growth of Pasteurella pestis, strain A1122, for the production of the envelope antigen (Englesberg and Levy, 1954), the potential use of this medium for plague toxin production became apparent. The development of a simplified method for the production of plague toxin made possible by this medium will be described here.
منابع مشابه
Selective Inhibition by Tryptophan Analogues of Murine Toxin Synthesis in Pasteurella Pestis.
Montie, Thomas C. (Albert Einstein Medical Center, Philadelphia, Pa.), and Samuel J. Ajl. Selective inhibition by tryptophan analogues of murine toxin synthesis in Pasteurella pestis. J. Bacteriol. 88:1467-1475. 1964.-Washed-cell suspensions of Pasteurella pestis, avirulent strain "Tjiwidej," exhibited a preferential inhibition of toxin synthesis relative to total protein formation, when grown ...
متن کاملStudies on plague. II. Immunological properties of purified Pasteurella pestis toxin.
The role played by the toxin of Pasteurella pestis in immunity against plague has been to date largely conjecture. The isolation of a highly purified toxin from this organism (Ajl et al., 1955) offers the necessary specific agent for the investigation of this problem. The studies to be described in this report were undertaken with a threefold purpose. They included (a) an attempt to manufacture...
متن کاملThe Identification and Isolation of Two Mouse-toxic Protein Components in Extracts from Pasteurella Pestis
The toxin activity of Pasteurella pestis cells, strain "Tjiwidej," was found to be associated with two proteins. Using a disc electrophoresis technique in conjunction with mouse lethality, two toxic proteins were isolated exhibiting intraperitoneal LD(50)'s of less than 1.0 to 1.5 microg protein. Each produced a single characteristic precipitin band on agar gel diffusion plates. The slower migr...
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Highly purified plague murine toxins A or B dissociate to form single uncommon polypeptide bands and another polypeptide band common to both toxins.
متن کاملThe murine toxin of Pasteurella pestis: a study in its development.
I NTRODUCTION................................................................ 177 ISOLATION AND PURIFICATION OF THETON .....................................T 178 IMMUNOLOGICAL PROPERTES .................................................... 179 MECHANIM OF ACTION OF THE ToXIN .......................................... 180 Effect of the Toxin on Microbial Extracts and Crude Tissue Homogenates........
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 68 1 شماره
صفحات -
تاریخ انتشار 1954